Recoverin Polyklonaler Antikörper

Recoverin Polyklonal Antikörper für WB, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Rezensionen (1)

Kat-Nr. : 10073-1-AP

Synonyme

RCVRN, Cancer associated retinopathy protein, Cancer-associated retinopathy protein, Protein CAR, RCV1

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  WB analysis using 10073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10073-1-AP (Recoverin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 10073-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10073-1-AP (RCVRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat retina using 10073-1-AP

  rat retina tissue were subjected to SDS PAGE followed by western blot with 10073-1-AP (Recoverin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse eye using 10073-1-AP

  IP result of anti-Recoverin (IP:10073-1-AP, 4ug; Detection:10073-1-AP 1:1000) with mouse eye tissue lysate 2680 ug.

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  IF Staining of mouse brain using 10073-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Recoverin antibody (10073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 10073-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Recoverin antibody (10073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, Ratten-Augengewebe, Ratten-Retina-Gewebe, Y79-Zellen
• Erfolgreiche IP: Maus-Augengewebe
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 12 publications below

Produktinformation

10073-1-AP bindet in WB, IHC, IF-P, IP, ELISA Recoverin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Recoverin fusion protein Ag0119
• Vollständiger Name: recoverin
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC001720
• Gene symbol: Recoverin
• Gene ID (NCBI): 5957
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Recoverin, belonging to a family of the neuronal calcium sensor (NCS) proteins, has a restricted expression in retinal photoreceptors or neurons or neuroendocrine cells. It has been suggested to play a role in light and dark adaptation by regulating rhodopsin phosphorylation. Recently, it has been found that autoantibodies against recoverin (24 kDa) have been strongly associated with cancer -associated retinopathy (CAR) syndrome, a paraneoplastic disease of the retina. But functions of recoverin in cancer cells remain unknown.

Publikationen

Species	Application	Title
rat	WB	J Neurosci; Differentiation of marrow stromal cells into photoreceptors in the rat eye.; Authors - Kicic Anthony A
mouse	WB	iScience; Identification of early-onset photoreceptor degeneration in transgenic mouse models of Alzheimer's disease.; Authors - Jie Zhang
human	IF	iScience; A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.; Authors - Suguru Yamasaki
human	IF	iScience; One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors.; Authors - Yuki Otsuka
human	IF	Sci Rep; Preconditioning the Initial State of Feeder-free Human Pluripotent Stem Cells Promotes Self-formation of Three-dimensional Retinal Tissue.; Authors - Atsushi Kuwahara
mouse	IF	Sci Rep; Microglia dynamics in retinitis pigmentosa model: formation of fundus whitening and autofluorescence as an indicator of activity of retinal degeneration.; Authors - Kenichi Makabe

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ROs

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