RDM1 Monoklonaler Antikörper

RDM1 Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

CloneNo.

1C10B4

Kat-Nr. : 66941-1-Ig

Synonyme

RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 66941-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66941-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66941-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66941-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 66941-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 66941-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66941-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66941-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 66941-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using RDM1 antibody (66941-1-Ig, Clone: 1C10B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66941-1-Ig bindet in WB, IHC, IF,ELISA RDM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RDM1 fusion protein Ag28686
• Vollständiger Name: RAD52 motif 1
• Berechnetes Molekulargewicht: 284 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC038301
• Gene symbol: RDM1
• Gene ID (NCBI): 201299
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)