REEP6 Polyklonaler Antikörper

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• KD/KO Validated

REEP6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12088-1-AP

Synonyme

Receptor expression-enhancing protein 6, Polyposis locus protein 1-like 1, DP1L1, C19orf32

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  WB analysis of BxPC-3 using 12088-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12088-1-AP (REEP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 12088-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12088-1-AP (REEP6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 12088-1-AP

  IP result of anti-REEP6 (IP:12088-1-AP, 4ug; Detection:12088-1-AP 1:600) with BxPC-3 cells lysate 2080 ug.

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  IHC staining of human pancreas cancer using 12088-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12088-1-AP (REEP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12088-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12088-1-AP (REEP6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12088-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using REEP6 antibody (12088-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of mouse olfactory epithelium tissue using 12088-1-AP

  Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of REEP6 antibody (12088-1-AP, 1:100). Excellent staining of Sus cells on the apical layer as well as their basal projections. By Dr. Brian Lin (Schwob Lab).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, Maus-Riechepithelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

12088-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA REEP6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: REEP6 fusion protein Ag2723
• Vollständiger Name: receptor accessory protein 6
• Berechnetes Molekulargewicht: 201 aa, 22 kDa
• Beobachtetes Molekulargewicht: 17-22 kDa
• GenBank-Zugangsnummer: BC008201
• Gene symbol: REEP6
• Gene ID (NCBI): 92840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IP	Mol Psychiatry; Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.; Authors - M G Sharoar
rat	WB	J Clin Invest; Nasal neuron PET imaging quantifies neuron generation and degeneration.; Authors - Genevieve C Van de Bittner
human	WB,IF	Am J Hum Genet; Mutations in REEP6 Cause Autosomal-Recessive Retinitis Pigmentosa.; Authors - Gavin Arno
mouse	IF	Hum Mol Genet; REEP6 deficiency leads to retinal degeneration through disruption of ER homeostasis and protein trafficking.; Authors - Smriti A Agrawal
human	WB,IHC	Int J Biol Macromol; Receptor accessory protein 6, a novel ferroptosis suppressor, drives oral squamous cell carcinoma by maintaining endoplasmic reticulum hemostasis; Authors - Lingyun Xia