RELB Polyklonaler Antikörper

RELB Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 25027-1-AP

Synonyme

I REL, IREL, RELB, Transcription factor RelB

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Galerie der Validierungsdaten

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  WB analysis using 25027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25027-1-AP (RELB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 25027-1-AP

  IP Result of anti-RELB (IP:25027-1-AP, 3ug; Detection:25027-1-AP 1:300) with Raji cells lysate 2500ug.

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  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 25027-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RELB antibody (25027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 25027-1-AP

  Immunofluorescent analysis of HeLa cells using 25027-1-AP (RELB antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of Jurkat using 25027-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human RELB (25027-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Maus-Thymusgewebe, Ramos-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

25027-1-AP bindet in WB, IP, IHC, IF, FC, ELISA RELB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RELB fusion protein Ag13824
• Vollständiger Name: v-rel reticuloendotheliosis viral oncogene homolog B
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62-65 kDa
• GenBank-Zugangsnummer: BC028013
• Gene symbol: RELB
• Gene ID (NCBI): 5971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Publikationen

Species	Application	Title
human	WB, IHC	Nat Genet; Exome sequencing identifies somatic mutations of DDX3X in natural killer/T-cell lymphoma.; Authors - Lu Jiang
human	IF	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
human	WB	Cell Death Dis; Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.; Authors - Xinhua Xiao
human	WB	J Clin Endocrinol Metab; Circulating Myeloid-derived Suppressor Cells Facilitate Invasion of Thyroid Cancer Cells by Repressing miR-486-3p.; Authors - Li Chen
human	FC	Front Oncol; NF-κB fingerprinting reveals heterogeneous NF-κB composition in diffuse large B-cell lymphoma; Authors - Eleanor Jayawant
mouse,human	WB	Antioxidants (Basel); Chlorogenic Acid Alleviated AFB1-Induced Hepatotoxicity by Regulating Mitochondrial Function, Activating Nrf2/HO-1, and Inhibiting Noncanonical NF-κB Signaling Pathway; Authors - Qianqian Wang