RELB Rekombinanter Antikörper

RELB Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242336G4

Kat-Nr. : 83305-6-RR

Synonyme

Transcription factor RelB, I-Rel, 242336G4

---

• 

  WB analysis using 83305-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83305-6-RR (RELB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Raji using 83305-6-RR

  1x10^6 Raji cells were intracellularly stained with 0.25 ug RELB Recombinant antibody (83305-6-RR, Clone:242336G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83305-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83305-6-RR against Human RELB were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in FC (Intra): Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83305-6-RR bindet in WB, FC (Intra), ELISA RELB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RELB fusion protein Ag13824
• Vollständiger Name: v-rel reticuloendotheliosis viral oncogene homolog B
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62-65 kDa
• GenBank-Zugangsnummer: BC028013
• Gene symbol: RELB
• Gene ID (NCBI): 5971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.