RELN Polyklonaler Antikörper

RELN Polyklonal Antikörper für IF, IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (2)

Kat-Nr. : 20689-1-AP

Synonyme

PRO1598, reelin, RELN, RL

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Galerie der Validierungsdaten

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  IHC staining of human liver cancer using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20689-1-AP (RELN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20689-1-AP (RELN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20689-1-AP (RELN Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human liver using 20689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20689-1-AP (RELN Antibody) at dilution of 1:50(under 40x lens).

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  IF Staining of mouse brain using 20689-1-AP

  Dual immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20689-1-AP (RELN antibody) at dilution of 1:50 and 66564-1-Ig (Tbr1 antibody), further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 20689-1-AP and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66564-1-Ig.

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  IF Staining of mouse brain using 20689-1-AP

  Dual immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20689-1-AP (RELN antibody) at dilution of 1:50 and 66564-1-Ig (Tbr1 antibody), further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 20689-1-AP and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66564-1-Ig.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

20689-1-AP bindet in IHC, IF, ELISA RELN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: reelin
• Berechnetes Molekulargewicht: 388 kDa
• GenBank-Zugangsnummer: NM_005045
• Gene symbol: RELN
• Gene ID (NCBI): 5649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RELN belongs to the reelin family. It is an extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. It is a secreted glycoprotein which is essential for neuronal migration in the cortex. RELN regulates microtubule function in neurons and neuronal migration. It affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2, RELN induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2).

Publikationen

Species	Application	Title
pig	IF	Sci Bull (Beijing); Single-cell dynamics of liver development in postnatal pigs; Authors - Lin Rao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

I tried RELN (in red) in human paraffin brain sections and the result was a very good specificity.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

RELN Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:20689-1-AP)

FH

Etienne (Verified Customer) (12-19-2022)

Worked very well at 1/50 and 1/200

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50, 1/200, 1/500
• Cell Tissue Type: Human Brain Organoid

RELN Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoid (Cat no:20689-1-AP)