Renin Polyklonaler Antikörper

Renin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Kat-Nr. : 14291-1-AP

Synonyme

REN, Angiotensinogenase, EC:3.4.23.15

---

• 

  WB analysis using 14291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14291-1-AP (Renin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14291-1-AP (Renin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human gliomas using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14291-1-AP (Renin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14291-1-AP (Renin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14291-1-AP (Renin antibody at dilution of 1:200.

• 

  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14291-1-AP (Renin antibody at dilution of 1:200.

• 

  IF Staining of HepG2 using 14291-1-AP

  Immunofluorescent analysis of HepG2 cells, using REN antibody 14291-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 14291-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Renin antibody (14291-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 12 publications below
• IF: See 4 publications below

Produktinformation

14291-1-AP bindet in WB, IHC, IF/ICC, ELISA Renin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Renin fusion protein Ag5612
• Vollständiger Name: renin
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC047752
• Gene symbol: Renin
• Gene ID (NCBI): 5972
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REN(Renin) is also named as angiotensinogenase and belongs to the peptidase A1 family. It is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Human prorenin and renin are synthesized in juxtaglomerular cells and it locates in the juxtaglomerular cells and afferent arteriole as cytoplasmic granules (PMID:19664745, 9453303). REN has 2 isoforms produced by alternative splicing with the molecular weight of 45-47kDa. The mature REN can be detected as 38-42 kDa due to the cleavage of signal peptide and propeptide, and also detected as 55-60 kDa with variable degrees of glycosylation (PMID: 20966072, 15226276).Defects in REN are a cause of renal tubular dysgenesis (RTD) and familial juvenile hyperuricemic nephropathy type 2 (HNFJ2).

Publikationen

Species	Application	Title
human	WB	Br J Pharmacol; Novel inhibitors of the cellular RAS components, Poricoic acids, target Smad3 phosphorylation and Wnt/β-catenin pathway against renal fibrosis.; Authors - Ming Wang
	WB	Plant Physiol; Lysine malonylation of DgnsLIPID TRANSFER PROTEIN1 at the K81 site improves cold resistance in chrysanthemum; Authors - Xiaoqin Liao
mouse	IHC	Hypertension; Dysregulation of Aldosterone Secretion in Mast Cell-Deficient Mice.; Authors - Hadrien-Gaël Boyer
rat	IHC	Antioxid Redox Signal; Silencing of central (Pro)renin receptor ameliorates salt-induced renal injury in CKD.; Authors - Jiawen Li
human	WB	J Agric Food Chem; Novel RAS Inhibitors Poricoic Acid ZG and Poricoic Acid ZH Attenuate Renal Fibrosis via a Wnt/β-Catenin Pathway and Targeted Phosphorylation of smad3 Signaling.; Authors - Ming Wang
mouse,human	WB,IF	Int J Mol Sci; Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions; Authors - Ning Liu