RFC3 Polyklonaler Antikörper

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• KD/KO Validated

RFC3 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 11814-1-AP

Synonyme

RF-C 38 kDa subunit

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• 

  WB analysis of human liver using 11814-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11814-1-AP (RFC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11814-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11814-1-AP (RFC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11814-1-AP (RFC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11814-1-AP

  IP result of anti-RFC3 (IP:11814-1-AP, 4 ug; Detection:11814-1-AP 1:600) with HeLa cells lysate 1200 ug.

• 

  IF Staining of U2OS using 11814-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC3 antibody (11814-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of U2OS using 11814-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC3 antibody (11814-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HeLa-Zellen, humanes Herzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

11814-1-AP bindet in WB, IF/ICC, IP, CoIP, ELISA RFC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFC3 fusion protein Ag2269
• Vollständiger Name: replication factor C (activator 1) 3, 38kDa
• Berechnetes Molekulargewicht: 40 aa, 6 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000149
• Gene symbol: RFC3
• Gene ID (NCBI): 5983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase . In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3 and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion and metastasis. RFC3 exists two isoforms with molecular weight of 34 kDa and 38-40 kDa.

Publikationen

Species	Application	Title
human	WB	Acta Biochim Biophys Sin (Shanghai); FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer; Authors - Wei Wang
human	WB	Virol J; IRF2 inhibits ZIKV replication by promoting FAM111A expression to enhance the host restriction effect of RFC3.; Authors - Kai Ren ; KD Validated
mouse	WB	Cell Rep; Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.; Authors - Stephen Gray
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang
human	CoIP	Exp Ther Med; RFC3 drives the proliferation, migration, invasion and angiogenesis of colorectal cancer cells by binding KIF14; Authors - Rong Yu