RFFL Polyklonaler Antikörper

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• KD/KO Validated

RFFL Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12687-1-AP

Synonyme

CARP 2, Caspase regulator CARP2, Fring, RFFL, RIFIFYLIN, RING finger protein 189, RING finger protein 34 like, RNF189, RNF34L

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  WB analysis of mouse testis using 12687-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12687-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12687-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12687-1-AP

  IP result of anti-RFFL (IP:12687-1-AP, 4ug; Detection:12687-1-AP 1:300) with mouse testis tissue lysate 3520 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12687-1-AP bindet in WB, IP, CoIP, ELISA RFFL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFFL fusion protein Ag3380
• Vollständiger Name: ring finger and FYVE-like domain containing 1
• Berechnetes Molekulargewicht: 327 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37-41 kDa
• GenBank-Zugangsnummer: BC028424
• Gene symbol: RFFL
• Gene ID (NCBI): 117584
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková
human	WB,CoIP	Cell Death Dis; ETS-1-activated LINC01016 over-expression promotes tumor progression via suppression of RFFL-mediated DHX9 ubiquitination degradation in breast cancers; Authors - Ying Sun ; KD Validated