RFK Polyklonaler Antikörper

RFK Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 15813-1-AP

Synonyme

Flavokinase, RFK, riboflavin kinase, RIFK

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15813-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15813-1-AP (RFK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15813-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15813-1-AP (RFK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 15813-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15813-1-AP (RFK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

15813-1-AP bindet in WB, IF, ELISA RFK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFK fusion protein Ag8591
• Vollständiger Name: riboflavin kinase
• Berechnetes Molekulargewicht: 162 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC007069
• Gene symbol: RFK
• Gene ID (NCBI): 55312
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.

Publikationen

Species	Application	Title
mouse	WB	Transl Psychiatry; Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice.; Authors - Michail S Kukharsky
mouse	WB	Eur J Immunol; Riboflavin (vitamin B2 ) deficiency impairs Nox2 priming and defense against Listeria monocytogenes.; Authors - Schramm Michael M
mouse	WB	Adv Sci (Weinh); Biomimetic Remodeling of Microglial Riboflavin Metabolism Ameliorates Cognitive Impairment by Modulating Neuroinflammation; Authors - Mengran Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (01-21-2023)

Works well in mouse tissue for IHC and WB. Standard antigen retrieval procedure and DAB IHC detection were used in the mouse spinal cord (overnight incubation at 4C). The protein primarily neuronal expression, showing weak but clean and clear signal. WB was done with semi-dry transfer and 4% milk in TBST.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse CNS tissue

RFK Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Mouse CNS tissue (Cat no:15813-1-AP)