RINT1 Polyklonaler Antikörper

RINT1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14567-1-AP

Synonyme

HsRINT 1, RAD50 interacting protein 1, RAD50 interactor 1, RINT 1, RINT1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 14567-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14567-1-AP (RINT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14567-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14567-1-AP (RINT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14567-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14567-1-AP (RINT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14567-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14567-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14567-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14567-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 14567-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 14567-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14567-1-AP

  Immunofluorescent analysis of HepG2 cells using 14567-1-AP (RINT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

14567-1-AP bindet in WB, IHC, IF, ELISA RINT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RINT1 fusion protein Ag6145
• Vollständiger Name: RAD50 interactor 1
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC068483
• Gene symbol: RINT1
• Gene ID (NCBI): 60561
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RINT-1 was first identified as a Rad50-interacting protein that participates in radiation-induced G2/M checkpoint control. It also involved in regulation of membrane traffic between the endoplasmic reticulum and the Golgi. It interacts with p130, an Rb family protein, and together they regulate the telomerase-independent telomere length. Otherwise, it interacts with Zeste white 10 with its N-terminal region throughout the cell cycle.

Publikationen

Species	Application	Title
human	IF	J Biol Chem; Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis; Authors - Robert S Kiss