RLBP1 Polyklonaler Antikörper

RLBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Rezensionen (1)

Kat-Nr. : 15356-1-AP

Synonyme

Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1

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  WB analysis using 15356-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15356-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15356-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 15356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 15356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15356-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Y79 using 15356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Retinal organoids using 15356-1-AP

  Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and CRALBP using 15356-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, HepG2-Zellen, Maus-Retina-Gewebe, Ratten-Augengewebe
• Erfolgreiche Detektion in IHC: Maus-Augengewebe, humanes Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe, Retinal organoids
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 13 publications below

Produktinformation

15356-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA RLBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RLBP1 fusion protein Ag7602
• Vollständiger Name: retinaldehyde binding protein 1
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC004199
• Gene symbol: RLBP1
• Gene ID (NCBI): 6017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

Publikationen

Species	Application	Title
zebrafish	IF	Aging Cell; Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.; Authors - Raquel R Martins
human	WB,IF	Mol Vis; Morphological, biochemical, and transcriptomic characterization of iPSC-derived human RPE cells from normal and Smith-Lemli-Opitz syndrome patients; Authors - Michael H Farkas
mouse	IF	Diabetes; CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy.; Authors - Jose-Andres C Portillo
mouse	WB	FASEB J; A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion.; Authors - Jose-Andres C Portillo
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
human	IF	Invest Ophthalmol Vis Sci; CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression; Authors - Sarah Vos

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval in NaCit 0.1M ph 6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Retina

RLBP1 Antibody Immunohistochemistry, validation (1:100 dilution) in Retina (Cat no:15356-1-AP)