RLBP1 Polyklonaler Antikörper

RLBP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (1)

Kat-Nr. : 15356-1-AP

Synonyme

CRALBP, RLBP1

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Galerie der Validierungsdaten

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  WB analysis using 15356-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15356-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Retinal organoids using 15356-1-AP

  Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and CRALBP using 15356-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, HepG2-Zellen, Maus-Retina-Gewebe, Ratten-Augengewebe
• Erfolgreiche Detektion in IHC: humanes Augengewebe, Maus-Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Retinal organoids

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 11 publications below

Produktinformation

15356-1-AP bindet in WB, IHC, IF, ELISA RLBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RLBP1 fusion protein Ag7602
• Vollständiger Name: retinaldehyde binding protein 1
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC004199
• Gene symbol: RLBP1
• Gene ID (NCBI): 6017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
zebrafish	IF	Aging Cell; Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.; Authors - Raquel R Martins
human	WB,IF	Mol Vis; Morphological, biochemical, and transcriptomic characterization of iPSC-derived human RPE cells from normal and Smith-Lemli-Opitz syndrome patients; Authors - Michael H Farkas
mouse	IF	Diabetes; CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy.; Authors - Jose-Andres C Portillo
mouse	WB	FASEB J; A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion.; Authors - Jose-Andres C Portillo
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
human	IF	Invest Ophthalmol Vis Sci; CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression; Authors - Sarah Vos

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval in NaCit 0.1M ph 6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Retina

RLBP1 Antibody Immunohistochemistry, validation (1:100 dilution) in Retina (Cat no:15356-1-AP)