RMI1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RMI1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 14630-1-AP

Synonyme

BLAP75, C9orf76, FAAP75, RMI1, RP11 346I8.1

---

• 

  WB analysis using 14630-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14630-1-AP (RMI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14630-1-AP

  WB result of RMI1 antibody (14630-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RMI1 transfected HepG2 cells.

• 

  IP experiment of HeLa using 14630-1-AP

  IP result of anti-RMI1 (IP:14630-1-AP, 3ug; Detection:14630-1-AP 1:1500) with HeLa cells lysate 3800ug.

• 

  IHC staining of human lung cancer using 14630-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14630-1-AP (RMI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14630-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14630-1-AP (RMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14630-1-AP bindet in WB, IHC, IF, IP, ELISA RMI1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RMI1 fusion protein Ag6208
• Vollständiger Name: RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC064937
• Gene symbol: RMI1
• Gene ID (NCBI): 80010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability. (PMID: 16537486, PMID: 16595695, PMID: 15775963)

Publikationen

Species	Application	Title
human	WB	Mol Cell; Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.; Authors - Thomas J Nicholls ; KD Validated
human	WB	Nat Commun; Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia.; Authors - Martin Moder ; KO Validated
	WB	Mol Ther; Improved Genome Editing through Inhibition of FANCM and Members of the BTR Dissolvase Complex.; Authors - Gustavo de Alencastro
human	WB	J Biol Chem; DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA-end resection in human cells.; Authors - Andreas Sturzenegger ; KD Validated
human	WB	J Cancer; Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p.; Authors - Jin Qian
human	WB	DNA Cell Biol; Identification and Bioinformatic Assessment of circRNA Expression After RMI1 Knockdown and Ionizing Radiation Exposure.; Authors - Yuxiao Sun