RNASEH1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RNASEH1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

41

Rezensionen (2)

Kat-Nr. : 15606-1-AP

Synonyme

H1RNA, Ribonuclease H type II, ribonuclease H1, RNase H1, RNASEH1, RNH1

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Galerie der Validierungsdaten

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  WB analysis using 15606-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15606-1-AP (RNASEH1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15606-1-AP

  IP Result of anti-RNASEH1 (IP:15606-1-AP, 3ug; Detection:15606-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 15606-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15606-1-AP (RNASEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, U2OS-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IF: See 17 publications below

Produktinformation

15606-1-AP bindet in WB, IP, IF, ELISA RNASEH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNASEH1 fusion protein Ag7990
• Vollständiger Name: ribonuclease H1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC002973
• Gene symbol: RNASEH1
• Gene ID (NCBI): 246243
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Publikationen

Species	Application	Title
human	WB	Nature; EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma.; Authors - Aparna Gorthi
human	WB	Cell; Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy.; Authors - Luciano E Marasco
human	WB	Nature; BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.; Authors - Vaibhav Bhatia ; KD Validated
human	WB	Cell Res; Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells.; Authors - Junyu Wu
mouse	IF	Nat Cell Biol; Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.; Authors - Flavia Michelini
human	WB	Mol Cell; METTL3 and N6-Methyladenosine Promote Homologous Recombination-Mediated Repair of DSBs by Modulating DNA-RNA Hybrid Accumulation.; Authors - Canfeng Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (10-05-2023)

Strong signal, very good detection.

• Applications: Western Blot

FH

Huai-Chin (Verified Customer) (06-08-2019)

Very good antibody. I've tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:3000 for Western, 1:500 for IHC, 1:200 for IF-IC
• Cell Tissue Type: Mammary gland/ MCF7