RNASEH2C Polyklonaler Antikörper

RNASEH2C Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16518-1-AP

Synonyme

AGS3, AYP1, Ribonuclease H2 subunit C, ribonuclease H2, subunit C, Ribonuclease HI subunit C, RNase H1 small subunit, RNase H2 subunit C, RNASEH2C

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Galerie der Validierungsdaten

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  WB analysis using 16518-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16518-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 16518-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16518-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16518-1-AP

  IP Result of anti-RNASEH2C (IP:16518-1-AP, 3ug; Detection:16518-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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  IHC staining of human brain using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Hodengewebe, humanes Milzgewebe, Jurkat-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Hautgewebe, humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

16518-1-AP bindet in WB, IP, IHC, ELISA RNASEH2C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNASEH2C fusion protein Ag9739
• Vollständiger Name: ribonuclease H2, subunit C
• Berechnetes Molekulargewicht: 164 aa, 18 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC023588
• Gene symbol: RNASEH2C
• Gene ID (NCBI): 84153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.; Authors - Michal Zimmermann
human	WB	Hum Mol Genet; Reduction of hRNase H2 activity in Aicardi-Goutières Syndrome cells leads to replication stress and genome instability.; Authors - Sara Pizzi
human	WB	J Biol Chem; Analysis of ribonucleotide removal from DNA by human nucleotide excision repair.; Authors - Laura A Lindsey-Boltz