RNASET2 Polyklonaler Antikörper

RNASET2 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 13753-1-AP

Synonyme

RNase T2, bA514O12.3, EC:4.6.1.19, Ribonuclease 6, Ribonuclease T2

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  IHC staining of mouse spleen using 13753-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 13753-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of transfected cells using 13753-1-AP

  IF result of 13753-1-AP (anti-RNASET2) in COS7 cell by Dr. Nancy Kedersha. COS7 cells transiently transfected with FAST-KD4-YFP, counterstained with PTG RNASET2 antibody (red) and human anti-mitochondrial antigen in blue. Note that FAST-KD4-YFP overexpression alters mitchonrial morphology, and is also present at stress granules (green only).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Transfizierte Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

13753-1-AP bindet in IHC, IF/ICC, ELISA RNASET2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNASET2 fusion protein Ag4695
• Vollständiger Name: ribonuclease T2
• Berechnetes Molekulargewicht: 256 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC039713
• Gene symbol: RNase T2
• Gene ID (NCBI): 8635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).

Publikationen

Species	Application	Title
human		FEBS J; Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import.; Authors - Stefan Schorr
human	IHC	Cell Transplant; Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer.; Authors - Mi Tian
mouse		Cell Signal; Musashi-2 binds with Fbxo6 to induce Rnaset2 ubiquitination and chemokine signaling pathway during vascular smooth muscle cell phenotypic switch in atherosclerosis; Authors - Tao Zhang
human		Arch Insect Biochem Physiol; Exploring the role of RNASET2 in the immune response of black soldier fly larvae; Authors - Sara Caramella
human	IHC	Adv Sci (Weinh); RNASET2 Deficiency Induces Hepatocellular Carcinoma Metastasis through Cholesterol-Triggered MET Activation; Authors - Yanquan Xu
human		Nat Commun; PMID: 30842415; Authors - Juanjuan Ou