RNF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RNF2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 16031-1-AP

Synonyme

HIP2 interacting protein 3, EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1

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  WB analysis of K-562 using 16031-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16031-1-AP

  WB result of RNF2 antibody (16031-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF2 transfected HEK-293 cells.

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  WB analysis of U-937 using 16031-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16031-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16031-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16031-1-AP

  IP result of anti-RNF2 (IP:16031-1-AP, 4ug; Detection:16031-1-AP 1:800) with K-562 cells lysate 1200ug.

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  IF Staining of HeLa using 16031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16031-1-AP (RNF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, humanes Hirngewebe, NIH/3T3-Zellen, U-937-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

16031-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA RNF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF2 fusion protein Ag8900
• Vollständiger Name: ring finger protein 2
• Berechnetes Molekulargewicht: 336 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC012583
• Gene symbol: RNF2
• Gene ID (NCBI): 6045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF2 (also known as ding, Ring1B or Ring2) is a member of the Ring finger protein family, which functions as E3 ubiquitin ligase for monoubiquitination of histone H2A at lysine 119 (H2AK119ub). Many studies have demonstrated that overexpressed RNF2 was involved in the pathological progression of multiple cancers and has an impact on their clinical features. For instance, the upregulated expression level of RNF2 is positively correlated with the occurrence and progression of hepatocellular carcinoma, melanoma, prostate cancer, breast cancer, pancreatic cancer, gastric cancer, and bladder urothelial carcinoma, as well as with the radioresistance of lung cancer and chemoresistance of ovarian cancer (PMID: 33400276). The molecular weight of RNF2 is 38 kDa.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Coordinated regulation of polycomb group complexes through microRNAs in cancer.; Authors - Qi Cao ; KD Validated
human	IHC	Cancer Cell; The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression.; Authors - Wenjing Su ; KD Validated
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
human	WB,IF	J Cell Biol; DUX4-induced HSATII transcription causes KDM2A/B-PRC1 nuclear foci and impairs DNA damage response; Authors - Tessa Arends ; KD Validated
human	WB,IHC	FEBS J; Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy; Authors - Yan Wei
human	WB, IF	Front Cell Dev Biol; RNF2 Mediates Hepatic Stellate Cells Activation by Regulating ERK/p38 Signaling Pathway in LX-2 Cells.; Authors - Qi Yan ; KD Validated