RPAP1 Monoklonaler Antikörper

RPAP1 Monoklonal Antikörper für WB, ELISA, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA, FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

2A6H5

Kat-Nr. : 68193-1-Ig

Synonyme

RPAP 1, RNA polymerase II-associated protein 1, KIAA1403, 2A6H5

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  WB analysis using 68193-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68193-1-Ig (RPAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 68193-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68193-1-Ig (RPAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 68193-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug RPAP1 Monoclonal antibody (68193-1-Ig, Clone:2A6H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68193-1-Ig bindet in WB, ELISA, FC (Intra) RPAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPAP1 fusion protein Ag7856
• Vollständiger Name: RNA polymerase II associated protein 1
• Berechnetes Molekulargewicht: 153 kDa
• Beobachtetes Molekulargewicht: 153 kDa
• GenBank-Zugangsnummer: BC000246
• Gene symbol: RPAP1
• Gene ID (NCBI): 26015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.