RPGR Polyklonaler Antikörper

RPGR Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16891-1-AP

Synonyme

COD1, CORDX1, CRD, orf15, PCDX, RP15, RP3, RPGR, XLRP3

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Galerie der Validierungsdaten

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  WB analysis of mouse eye using 16891-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16891-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 16891-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 16891-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 16891-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 16891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 16891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16891-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16891-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16891-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16891-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 16891-1-AP

  IF result of anti-RPGR (16891-1-AP) in hTERT-RPE1 cell by Dr. Seongjin Seo.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, Mauslebergewebe, Maus-Retina-Gewebe, SH-SY5Y-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: Maus-Augengewebe, humanes Herzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16891-1-AP bindet in WB, IHC, IF, ELISA RPGR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPGR fusion protein Ag10405
• Vollständiger Name: retinitis pigmentosa GTPase regulator
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 100-105 kDa, 70 kDa
• GenBank-Zugangsnummer: BC031624
• Gene symbol: RPGR
• Gene ID (NCBI): 6103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPGR is a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. This antibody can recognize all the isoforms of RPGR.

Publikationen

Species	Application	Title
human	WB	Elife; Multiple ciliary localization signals control INPP5E ciliary targeting; Authors - Dario Cilleros-Rodriguez
human	WB	J Pers Med; Human iPSC-Derived Retinal Organoids and Retinal Pigment Epithelium for Novel Intronic RPGR Variant Assessment for Therapy Suitability.; Authors - Fidelle Chahine Karam