RPGRIP1L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPGRIP1L Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Rezensionen (1)

Kat-Nr. : 55160-1-AP

Synonyme

CORS3, FTM, JBTS7, KIAA1005, MKS5, Protein fantom, RPGRIP1 like, RPGRIP1 like protein, RPGRIP1L

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  WB analysis of HEK-293 using 55160-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55160-1-AP (RPGRIP1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 55160-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 55160-1-AP (RPGRIP1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 55160-1-AP

  IP result of anti-RPGRIP1L (IP:55160-1-AP, 4ug; Detection:55160-1-AP 1:700) with HEK-293 cells lysate 3200ug.

• 

  IHC staining of human pancreas using 55160-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55160-1-AP (RPGRIP1L antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 55160-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55160-1-AP (RPGRIP1L antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 55160-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55160-1-AP (RPGRIP1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 55160-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55160-1-AP (RPGRIP1L antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Nierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

55160-1-AP bindet in WB, IHC, IP, CoIP, ELISA RPGRIP1L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hund, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RPGRIP1-like
• Berechnetes Molekulargewicht: 151 kDa
• GenBank-Zugangsnummer: NM_015272
• Gene symbol: RPGRIP1L
• Gene ID (NCBI): 23322
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPGRIP1L, also named as FTM, KIAA1005, belongs to the RPGRIP1 family. It negatively regulates signaling through the G-protein coupled thromboxane A2 receptor. RPGRIP1L may be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis.(PMID:17558409) Defects in RPGRIP1L are the cause of Joubert syndrome type 7 (JBTS7). Defects in RPGRIP1L are the cause of Meckel syndrome type 5 (MKS5). This antibody is specific to RPGRIP1L.

Publikationen

Species	Application	Title
mouse		Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu
		Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu
human		Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human		Am J Hum Genet; Mutations in CSPP1, Encoding a Core Centrosomal Protein, Cause a Range of Ciliopathy Phenotypes in Humans.; Authors - Ranad Shaheen
		J Cell Biol; TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone.; Authors - Elle C Roberson
		Dev Cell; Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.; Authors - Quynh P H Nguyen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alice (Verified Customer) (01-25-2019)

Cells fixed in MeOH at -20'C for 5mins.Blocked with 1% w/v Milk/PBSRPGRIP1L antibody diluted in block 1:1000 and co-stained with Acetylated alpha-Tubulin and gamma-tubulin.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hTERT RPE-1

RPGRIP1L Antibody Immunofluorescence, validation (1:1000 dilution) in hTERT RPE-1 (Cat no:55160-1-AP)