RPL10 Polyklonaler Antikörper

RPL10 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17013-1-AP

Synonyme

60S ribosomal protein L10, DXS648, DXS648E, Laminin receptor homolog, NOV, Protein QM, QM, ribosomal protein L10, RPL10, Tumor suppressor QM

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Galerie der Validierungsdaten

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  WB analysis using 17013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17013-1-AP bindet in WB, IHC, IF, ELISA RPL10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL10 fusion protein Ag9969
• Vollständiger Name: ribosomal protein L10
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC003358
• Gene symbol: RPL10
• Gene ID (NCBI): 6134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPL10, also named as Large ribosomal subunit protein uL16, is a 214 amino acid protein, which belongs to the universal ribosomal protein uL16 family. RPL10 is a component of the large ribosomal subunit and is down-regulated during adipocyte, kidney, and heart differentiation. RPL10 shares 98% amino acid sequence with RPL10L, so RPL10 antibody is able to recognize RPL10 and RPL10L protein.

Publikationen

Species	Application	Title
human	WB	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
human	WB,IHC	Transl Oncol; Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.; Authors - Xinlu Liu
mouse	IF	Development; Lin28-mediated promotion of protein synthesis is critical for neural progenitor cell maintenance and brain development in mice.; Authors - Stephanie Herrlinger
human	WB	Brain; GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation; Authors - Yu Fan
human	WB	Cardiovasc Res; The translational landscape of human vascular smooth muscle cells identifies novel short ORF-encoded peptide regulators for phenotype alteration; Authors - Kang Li