RPL10A Polyklonaler Antikörper

RPL10A Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, PLA, IP, IF, FC, CoIP

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 16681-1-AP

Synonyme

60S ribosomal protein L10a, Csa 19, NEDD 6, NEDD6, ribosomal protein L10a, RPL10A

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16681-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16681-1-AP (RPL10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16681-1-AP (RPL10A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16681-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16681-1-AP (RPL10A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16681-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16681-1-AP (RPL10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16681-1-AP

  IP Result of anti-RPL10A (IP:16681-1-AP, 4ug; Detection:16681-1-AP 1:700) with HeLa cells lysate 1600ug.

• 

  IF Staining of HepG2 using 16681-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL10A antibody 16681-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Lebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 2 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16681-1-AP bindet in WB, PLA, IP, IF, FC, CoIP RPL10A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Krallenfrosch (Xenopus)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL10A fusion protein Ag10009
• Vollständiger Name: ribosomal protein L10a
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC006791
• Gene symbol: RPL10A
• Gene ID (NCBI): 4736
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
Xenopus	WB	Neuron; Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.; Authors - Jean-Michel Cioni
mouse	IF	Nat Commun; Cilia locally synthesize proteins to sustain their ultrastructure and functions.; Authors - Kai Hao
human	FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	WB, PLA	Elife; Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.; Authors - Max Koppers
human	WB, CoIP	Adv Sci (Weinh); Dual Inhibition of DKC1 and MEK1/2 Synergistically Restrains the Growth of Colorectal Cancer Cells.; Authors - Guangyan Kan
Xenopus	WB,IF	Cell; Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.; Authors - Jean-Michel Cioni

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (11-22-2021)

this antibody is very good and didn't show non-specific bands in HEK293T cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

RPL10A Antibody Western Blot validation (1:2000 dilution) in (Cat no:16681-1-AP)