RPL12 Polyklonaler Antikörper

RPL12 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14536-1-AP

Synonyme

ribosomal protein L12, Large ribosomal subunit protein uL11, 60S ribosomal protein L12

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  WB analysis using 14536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14536-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14536-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 14536-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 14536-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 14536-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 14536-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 14536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14536-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 14536-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Endometriumkarzinomgewebe, Maushirngewebe, Maus-Magengewebe, Rattenhirngewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

14536-1-AP bindet in WB, IHC, IF, ELISA RPL12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hefe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL12 fusion protein Ag6037
• Vollständiger Name: ribosomal protein L12
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC050644
• Gene symbol: RPL12
• Gene ID (NCBI): 6136
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Biol; CDK1 couples proliferation with protein synthesis.; Authors - Katharina Haneke
human	WB	Sci Rep; RNA G-quadruplex structures control ribosomal protein production; Authors - DhavalVarshney
human,yeast,C.elegans	WB,IF	Nat Cell Biol; Rpl12 is a conserved ribophagy receptor; Authors - Yuting Chen ; KD Validated