RPL18 Polyklonaler Antikörper

RPL18 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 17029-1-AP

Synonyme

60S ribosomal protein L18, ribosomal protein L18, RPL18

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• 

  WB analysis of HeLa using 17029-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17029-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17029-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

17029-1-AP bindet in WB, ELISA RPL18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL18 fusion protein Ag10474
• Vollständiger Name: ribosomal protein L18
• Berechnetes Molekulargewicht: 188 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22-26 kDa
• GenBank-Zugangsnummer: BC009708
• Gene symbol: RPL18
• Gene ID (NCBI): 6141
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
pig	WB	Virus Res; Up-regulated 60S ribosomal protein L18 in PEDV N protein-induced S-phase arrested host cells promotes viral replication; Authors - Qinghe Zhu ; KD Validated
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk
mouse	WB	Biomaterials; P-Pev: micelle-like complexes transformed from tumor extracellular vesicles by PEG-PE for personalized therapeutic tumor vaccine; Authors - Hongjian Tian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katie (Verified Customer) (05-23-2022)

Gives appropriate sized band in western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T cells - mouse