RPL18A Polyklonaler Antikörper

RPL18A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 14653-1-AP

Synonyme

ribosomal protein L18a, Large ribosomal subunit protein eL20, 60S ribosomal protein L18a

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  WB analysis of HepG2 using 14653-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14653-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14653-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14653-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14653-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 14653-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 14653-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 14653-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL18A antibody (14653-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 14653-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL18A antibody 14653-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Hirngewebe, Mauslungengewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

14653-1-AP bindet in WB, IHC, IF/ICC, ELISA RPL18A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL18A fusion protein Ag6251
• Vollständiger Name: ribosomal protein L18a
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC066319
• Gene symbol: RPL18A
• Gene ID (NCBI): 6142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.

Publikationen

Species	Application	Title
human	WB	Sci Rep; Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.; Authors - Haiping Tang
mouse	WB	Genetics; Quantitative changes in gimap3 and gimap5 expression modify mitochondrial DNA segregation in mice.; Authors - Riikka Jokinen
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren
rat	WB	EMBO Rep; Molecular view of ER membrane remodeling by the Sec61/TRAP translocon; Authors - Sudeep Karki