RPL19 Polyklonaler Antikörper

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• KD/KO Validated

RPL19 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 14701-1-AP

Synonyme

60S ribosomal protein L19, Large ribosomal subunit protein eL19, ribosomal protein L19

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  WB analysis of HeLa using 14701-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14701-1-AP

  WB result of RPL19 antibody (14701-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL19 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 14701-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14701-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14701-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14701-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14701-1-AP

  IP result of anti-RPL19 (IP:14701-1-AP, 4ug; Detection:14701-1-AP 1:3000) with HeLa cells lysate 1560 ug.

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  IHC staining of human brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14701-1-AP (RPL19 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14701-1-AP (RPL19 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14701-1-AP (RPL19 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14701-1-AP (RPL19 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14701-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14701-1-AP (RPL19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14701-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human RPL19 (14701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, Mausmilzgewebe, Maushodengewebe, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

14701-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA RPL19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL19 fusion protein Ag6371
• Vollständiger Name: ribosomal protein L19
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC066315
• Gene symbol: RPL19
• Gene ID (NCBI): 6143
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPL19, also named as 60S ribosomal protein L19, is a 196 amino acid protein, which belongs to the ribosomal protein L19e family. RPL19 encodes a ribosomal protein that is a component of the 60S subunit.

Publikationen

Species	Application	Title
human	WB	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
mouse	WB	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
human	WB	Hum Mutat; Frameshift mutation in p53 regulator RPL26 is associated with multiple physical abnormalities and a specific pre-ribosomal RNA processing defect in diamond-blackfan anemia.; Authors - Gazda Hanna T HT
		EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans