RPL26L1 Polyklonaler Antikörper

RPL26L1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16487-1-AP

Synonyme

60S ribosomal protein L26-like 1, Large ribosomal subunit protein uL24-like 1, ribosomal protein L26 like 1, Ribosomal protein uL24-like, RPL26P1

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  WB analysis of HEK-293 using 16487-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16487-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16487-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16487-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 16487-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16487-1-AP (RPL26L1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16487-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPL26L1 antibody (16487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16487-1-AP bindet in WB, IHC, IF/ICC, ELISA RPL26L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL26L1 fusion protein Ag9609
• Vollständiger Name: ribosomal protein L26-like 1
• Berechnetes Molekulargewicht: 145 aa, 17 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC017360
• Gene symbol: RPL26L1
• Gene ID (NCBI): 51121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPL26L1, also named as RPL26P1, belongs to the universal ribosomal protein uL24 family. RPL26L1 shares high sequence similarity with ribosomal protein L26 and plays a role in protein synthesis (PMID: 31673979). The molecular mass of RPL26L1 is 17 kDa. Studies have shown that RPL26L1 is related to autism spectrum disorder and is one of the potential biomarkers of the neurodegenerative disease Friedreich's ataxia. In addition, RPL26L1 may be closely related with SLE development (PMID: 31889427).

Publikationen

Species	Application	Title
rat	WB	Brain Sci; Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest; Authors - Qiang Zhang