RPL27 Polyklonaler Antikörper

RPL27 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14980-1-AP

Synonyme

60S ribosomal protein L27, ribosomal protein L27, RPL27

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 14980-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14980-1-AP (RPL27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14980-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14980-1-AP (RPL27 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14980-1-AP (RPL27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14980-1-AP (RPL27 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

14980-1-AP bindet in WB, IHC, IF, ELISA RPL27 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL27 fusion protein Ag6893
• Vollständiger Name: ribosomal protein L27
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC001700
• Gene symbol: RPL27
• Gene ID (NCBI): 6155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPL27, also named as 60S ribosomal protein L27, is a 136 amino acid protein, which contains one KOW domain and belongs to the ribosomal protein L27e family. The function of RPL27 is still non-known. RPL27 may play a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome.

Publikationen

Species	Application	Title
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
rat	WB,IHC	J Orthop Translat; Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression.; Authors - Zhenyuan Wei
rat	WB	Physiol Rep; Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules.; Authors - Muhammad U Cheema
mouse	WB	EMBO Rep; miR-132 suppresses transcription of ribosomal proteins to promote protective Th1 immunity.; Authors - James P Hewitson
rat	WB	Brain Sci; Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest; Authors - Qiang Zhang
mouse	IF	Nat Struct Mol Biol; Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment; Authors - Dingfeng Li