RPL29 Polyklonaler Antikörper

RPL29 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (2)

Kat-Nr. : 15799-1-AP

Synonyme

ribosomal protein L29, Large ribosomal subunit protein eL29, HUMRPL29, HIP, Cell surface heparin-binding protein HIP

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  WB analysis using 15799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 15799-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL29 antibody (15799-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HEK-293T-Zellen, HepG2-Zellen, HUVEC-Zellen, Maus-Pankreasgewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

15799-1-AP bindet in WB, IHC, IF/ICC, ELISA RPL29 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL29 fusion protein Ag8510
• Vollständiger Name: ribosomal protein L29
• Berechnetes Molekulargewicht: 159 aa, 18 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC008926
• Gene symbol: RPL29
• Gene ID (NCBI): 6159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).The antibody can detect the band around 22kDa.

Publikationen

Species	Application	Title
human	WB	Nature; Systematic quantitative analysis of ribosome inventory during nutrient stress.; Authors - Heeseon An
mouse	WB	J Cell Sci; Germline Deletion of Huntingtin Causes Male Infertility and Arrested Spermiogenesis in Mice.; Authors - Jinting Yan
human,mouse	WB	Sci Rep; The tissue specific regulation of miR22 expression in the lung and brain by ribosomal protein L29.; Authors - Mohammad Ishaque Ali
human	WB	Sci Adv; Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells; Authors - Chandreyee Datta
		Nucleic Acids Res; ADAR3 modulates neuronal differentiation and regulates mRNA stability and translation; Authors - Victor Karlström
human	WB	Mol Cell; RIOK3 mediates the degradation of 40S ribosomes; Authors - Zixuan Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

A great antibody with strong signal and specificity. We confirmed this antibody in HCT116 and HEK293T cell lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

FH

H (Verified Customer) (04-05-2020)

This antibody works very well. I used this antibody when screening clones for CRISPR knock-in.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

RPL29 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:15799-1-AP)