Ribosomal protein L4 Polyklonaler Antikörper

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• KD/KO Validated

Ribosomal protein L4 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(40)

Rezensionen (1)

Kat-Nr. : 11302-1-AP

Synonyme

RPL4, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4, RPL1

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  WB analysis using 11302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11302-1-AP

  WB result of Ribosomal protein L4 antibody (11302-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HeLa cells.

• 

  WB analysis of rat skeletal muscle using 11302-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11302-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11302-1-AP

  IP result of anti-Ribosomal protein L4 (IP:11302-1-AP, 4ug; Detection:11302-1-AP 1:6000) with HeLa cells lysate 1050 ug.

• 

  IHC staining of human lymphoma using 11302-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11302-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ribosomal protein L4 antibody (11302-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HeLa using 11302-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Ribosomal protein L4 (11302-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 38 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

11302-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA Ribosomal protein L4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, arabidopsis, Maus, Ratte, Reis
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ribosomal protein L4 fusion protein Ag1828
• Vollständiger Name: ribosomal protein L4
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC009888
• Gene symbol: Ribosomal protein L4
• Gene ID (NCBI): 6124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

Publikationen

Species	Application	Title
	WB	Science; The Ccr4-Not complex monitors the translating ribosome for codon optimality.; Authors - Robert Buschauer
human	WB	Cell; Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate.; Authors - Colin Chih-Chien Wu
mouse	IF	Cell Res; Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.; Authors - Xiaochen Gai
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human	WB	Mol Cell; N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.; Authors - Jun Zhou
human	WB	Mol Cell; The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation.; Authors - Cindy Meyer

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anita (Verified Customer) (10-30-2020)

1:1000 dilution works for HeLa cells, not for C. elegans nematodes

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

Ribosomal protein L4 Antibody Western Blot validation (1:1000 dilution) in (Cat no:11302-1-AP)