RPL7 Polyklonaler Antikörper

RPL7 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14583-1-AP

Synonyme

60S ribosomal protein L7, humL7 1, ribosomal protein L7, RPL7

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 14583-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14583-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14583-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14583-1-AP

  IP Result of anti-RPL7 (IP:14583-1-AP, 3ug; Detection:14583-1-AP 1:1000) with HEK-293 cells lysate 880ug.

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  IHC staining of human skin cancer using 14583-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin cancer using 14583-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 14583-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 14583-1-AP (RPL7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Produktinformation

14583-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA RPL7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL7 fusion protein Ag6104
• Vollständiger Name: ribosomal protein L7
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29-30 kDa
• GenBank-Zugangsnummer: BC006095
• Gene symbol: RPL7
• Gene ID (NCBI): 6129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].

Publikationen

Species	Application	Title
	WB	Nat Cell Biol; METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis.; Authors - Rui Su
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB,RIP	Biochem Biophys Res Commun; CRISPR-based RNA-binding protein mapping in live cells.; Authors - Baiwen Chen
human	WB	Front Endocrinol (Lausanne); Glucose controls co-translation of structurally related mRNAs via the mTOR and eIF2 pathways in human pancreatic beta cells; Authors - Manuel Bulfoni
human	WB	Science; Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function; Authors - Wonyul Jang
human	WB	Cell; QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism; Authors - Zhicong Zhao