RPLP0 Polyklonaler Antikörper

RPLP0 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 11290-2-AP

Synonyme

60S ribosomal protein L10E, L10E, P0, PRLP0, ribosomal protein, large, P0, RPLP0, RPP0

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Galerie der Validierungsdaten

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  WB analysis using 11290-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11290-2-AP (RPLP0 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11290-2-AP

  IP result of anti-RPLP0 (IP:11290-2-AP, 4ug; Detection:11290-2-AP 1:500) with HeLa cells lysate 2680 ug.

• 

  IP experiment of HeLa using 11290-2-AP

  IP Result of anti-RPLP0 (IP:11290-2-AP, 4ug; Detection:11290-2-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human colon cancer using 11290-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11290-2-AP (RPLP0 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 11290-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11290-2-AP (RPLP0 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 1 publications below
• ELISA: See 3 publications below
• RIP: See 1 publications below

Produktinformation

11290-2-AP bindet in WB, RIP, IP, IHC, IF, ELISA RPLP0 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPLP0 fusion protein Ag1829
• Vollständiger Name: ribosomal protein, large, P0
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC009867
• Gene symbol: RPLP0
• Gene ID (NCBI): 6175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPLP0 is first identified as a cytosolic protein which takes a part in the ribosomal stalk and interacts with RPLP1 and RPLP2. It also has potential roles in molecular transport from outside into the cells. It generally presents in multiple copies on the ribosome and have isoelectric points in the range of pH 3 to 5, in contrast to most ribosomal proteins, which are single copy and basic

Publikationen

Species	Application	Title
human	WB	Cell Metab; miR-378(∗) mediates metabolic shift in breast cancer cells via the PGC-1β/ERRγ transcriptional pathway.; Authors - Lillian J Eichner
human	WB	Genes Dev; The homeobox protein Prox1 is a negative modulator of ERR{alpha}/PGC-1{alpha} bioenergetic functions.; Authors - Charest-Marcotte Alexis A
human	WB	J Cell Biol; Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases.; Authors - Yun Chawon C
mouse	WB	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	PLoS Genet; Genomic convergence among ERRα, PROX1, and BMAL1 in the control of metabolic clock outputs.; Authors - Dufour Catherine R CR

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Trinh (Verified Customer) (06-02-2020)

good antibody

• Applications: Western Blot
• Cell Tissue Type: HUVEC