RPS12 Polyklonaler Antikörper

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• KD/KO Validated

RPS12 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 16490-1-AP

Synonyme

40S ribosomal protein S12, ribosomal protein S12, Small ribosomal subunit protein eS12

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  WB analysis of NIH/3T3 using 16490-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16490-1-AP

  WB result of RPS12 antibody (16490-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS12 transfected HepG2 cells.

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  WB analysis using 16490-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16490-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16490-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16490-1-AP

  IP result of anti-RPS12 (IP:16490-1-AP, 4ug; Detection:16490-1-AP 1:1000) with HeLa cells lysate 2920 ug.

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  IHC staining of human colon cancer using 16490-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16490-1-AP (RPS12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16490-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16490-1-AP (RPS12 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16490-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RPS12 antibody (16490-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

16490-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RPS12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS12 fusion protein Ag9615
• Vollständiger Name: ribosomal protein S12
• Berechnetes Molekulargewicht: 132 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC017321
• Gene symbol: RPS12
• Gene ID (NCBI): 6206
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.This antibody specifically recognizes the 15kd RPS12 protein.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity; Authors - Joanna R Kovalski
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
	WB	Dev Cell; Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions; Authors - Max Koppers
		Dev Cell; Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.; Authors - Kevin F Chau
mouse	IHC	Elife; Downregulation of ribosome biogenesis during early forebrain development.; Authors - Kevin F Chau
human	WB	Sci Signal; Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas; Authors - Matthew J Knarr