RPS15 Polyklonaler Antikörper

RPS15 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 14957-1-AP

Synonyme

40S ribosomal protein S15, ribosomal protein S15, RIG, RIG protein, RPS15

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Galerie der Validierungsdaten

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  WB analysis of human liver using 14957-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14957-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14957-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14957-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 14957-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14957-1-AP (RPS15 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14957-1-AP (RPS15 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse liver using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14957-1-AP

  Immunofluorescent analysis of Hela cells, using RPS15 antibody 14957-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HeLa-Zellen, L02-Zellen, Maushirngewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe, Mauslebergewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

14957-1-AP bindet in WB, IHC, IF, ELISA RPS15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS15 fusion protein Ag6795
• Vollständiger Name: ribosomal protein S15
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC064908
• Gene symbol: RPS15
• Gene ID (NCBI): 6209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS15, also named as RIG protein or Small ribosomal subunit protein uS19, is a 145 amino acid protein, which belongs to the universal ribosomal protein uS19 family. RPS15 is a ribosomal small subunit assembly and involved in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay.

Publikationen

Species	Application	Title
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
human	IF	Cell Prolif; An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms; Authors - Nan Zhang
human	WB	Elife; The molecular basis of coupling between poly(A)-tail length and translational efficiency.; Authors - Kehui Xiang
human		Cell Rep; FKBP10 Regulates Protein Translation to Sustain Lung Cancer Growth.; Authors - Giorgio Ramadori
human	WB	Elife; The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1.; Authors - Diego Acosta-Alvear
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Advait (Verified Customer) (02-22-2019)

4% PFA fixationBlocking buffer: BSA with 0.05% SaponinIncubate at 4 degrees overnight for best staining quality.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

RPS15 Antibody Western Blot,Immunofluorescence, validation (1:100 dilution) in HeLa (Cat no:14957-1-AP)