RPS20 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPS20 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 15692-1-AP

Synonyme

40S ribosomal protein S20, ribosomal protein S20, RPS20

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  WB analysis of MCF-7 using 15692-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15692-1-AP

  WB result of RPS20 antibody (15692-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS20 transfected HepG2 cells.

• 

  WB analysis of HepG2 using 15692-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15692-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15692-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

15692-1-AP bindet in WB, IHC, ELISA RPS20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS20 fusion protein Ag8257
• Vollständiger Name: ribosomal protein S20
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC007507
• Gene symbol: RPS20
• Gene ID (NCBI): 6224
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity; Authors - Joanna R Kovalski
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human	WB	bioRxiv; Functional screen for mediators of onco-mRNA translation specificity; Authors - Joanna R Kovalski
human,mouse	WB,IHC	Oncol Rep; Biochemical and clinical effects of RPS20 expression in renal clear cell carcinoma; Authors - Cheng Shen ; KD Validated
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren
human,mouse	WB	bioRxiv; RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages; Authors - Teodorus Theo Susanto