RPS20 Monoklonaler Antikörper

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• KD/KO Validated

RPS20 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

3E9B4

Kat-Nr. : 68270-1-Ig

Synonyme

3E9B4, 40S ribosomal protein S20, ribosomal protein S20, Small ribosomal subunit protein uS10

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  WB analysis using 68270-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 68270-1-Ig

  WB result of RPS20 antibody (68270-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS20 transfected HepG2 cells.

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  WB analysis using 68270-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 68270-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68270-1-Ig (RPS20 antibody) at dilution of 1:1000 (under 10x lens).

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  IF Staining of HeLa using 68270-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS20 antibody (68270-1-Ig, Clone: 3E9B4 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

68270-1-Ig bindet in WB, IHC, IF/ICC, ELISA RPS20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPS20 fusion protein Ag8275
• Vollständiger Name: ribosomal protein S20
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC007507
• Gene symbol: RPS20
• Gene ID (NCBI): 6224
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity; Authors - Joanna R Kovalski
human	WB	Sci Rep; Hydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis; Authors - Xuan He
human	IF	bioRxiv; Functional screen for mediators of onco-mRNA translation specificity; Authors - Joanna R Kovalski