RPS26 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPS26 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA, PLA

Konjugation

Unkonjugiert

Publikationen(14)

Rezensionen (1)

Kat-Nr. : 14909-1-AP

Synonyme

Small ribosomal subunit protein eS26, ribosomal protein S26, 40S ribosomal protein S26

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  WB analysis of mouse ovary using 14909-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14909-1-AP (RPS26 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14909-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14909-1-AP (RPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 14909-1-AP

  IP result of anti-RPS26 (IP:14909-1-AP, 4ug; Detection:14909-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human kidney using 14909-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14909-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14909-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14909-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14909-1-AP (RPS26 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 14909-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RPS26 antibody 14909-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

14909-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA, PLA RPS26 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS26 fusion protein Ag6706
• Vollständiger Name: ribosomal protein S26
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 18-21 kDa
• GenBank-Zugangsnummer: BC002604
• Gene symbol: RPS26
• Gene ID (NCBI): 6231
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia that is usually diagnosed during early infancy, and strikingly, in addition to defects in red cell maturation the disorder is associated with various physical anomalies in about 40% of patients. To date, heterozygous mutations in nine RP genes have been conclusively associated with DBA, and RPS26 is one of them. There are nine mutation found in RPS26 from DBA patients.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
		Nat Commun; Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity.; Authors - Yanqin Yu
human	WB	Nat Commun; Genome-wide search for exonic variants affecting translational efficiency.; Authors - Li Quan Q
human	IHC, WB	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB, PLA	Elife; Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.; Authors - Max Koppers
human	WB,IP	Oncogene; The ribosomal protein S26 regulates p53 activity in response to DNA damage.; Authors - Cui D D ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (09-24-2023)

The esophageal cancer cell line is used. The antibody works very stably, and the concentration can be from 1:1000 to 1:2000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500

RPS26 Antibody Western Blot validation (1:1500 dilution) in (Cat no:14909-1-AP)