RPS3 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

RPS3 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

CloneNo.

2G7H4

Kat-Nr. : 66046-1-Ig

Synonyme

40S ribosomal protein S3, Cell and organelle markers, ribosomal protein S3, Ribosome Marker, RPS3

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Galerie der Validierungsdaten

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  WB analysis using 66046-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66046-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66046-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66046-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66046-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66046-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66046-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66046-1-Ig

  IP Result of anti-RPS3 (IP:66046-1-Ig, 3ug; Detection:66046-1-Ig 1:2000) with HEK-293 cells lysate 1800ug.

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  IHC staining of human ovary tumor using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66046-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66046-1-Ig(RPS3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Jurkat-Zellen, K-562-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

66046-1-Ig bindet in WB, IP, IHC, IF, FC, ChIP, ELISA RPS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPS3 fusion protein Ag7515
• Vollständiger Name: ribosomal protein S3
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC003137
• Gene symbol: RPS3
• Gene ID (NCBI): 6188
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.

Publikationen

Species	Application	Title
human	FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	PLoS Pathog; Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery.; Authors - Benjamin Götte
human	WB	Free Radic Biol Med; Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.; Authors - Reiko Iida
human	WB	Oncotarget; Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated.; Authors - Yong Joong Kim ; KD Validated
human	WB,IF	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos