RPS4X Rekombinanter Antikörper

RPS4X Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242697E12

Kat-Nr. : 85040-5-RR

Synonyme

242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform

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  WB analysis using 85040-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85040-5-RR (SCAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 85040-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS4X antibody (85040-5-RR, Clone: 242697E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 85040-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS4X Recombinant antibody (85040-5-RR, Clone:242697E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85040-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85040-5-RR against Human RPS4X were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Maushirngewebe, Mausmilzgewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85040-5-RR bindet in WB, IF/ICC, FC (Intra), ELISA RPS4X und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RPS4X fusion protein Ag6513
• Vollständiger Name: ribosomal protein S4, X-linked
• Berechnetes Molekulargewicht: 263 aa, 30 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC000472
• Gene symbol: RPS4X
• Gene ID (NCBI): 6191
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). (PMID:8358435)