RPS4Y1 Polyklonaler Antikörper

RPS4Y1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17296-1-AP

Synonyme

Small ribosomal subunit protein eS4, Y isoform 1, RPS4Y, PRO2646, 40S ribosomal protein S4

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  WB analysis of DU 145 using 17296-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 17296-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 17296-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17296-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 17296-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 17296-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 17296-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17296-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 17296-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17296-1-AP (RPS4Y1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17296-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS4Y1 antibody (17296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, A2780-Zellen, HeLa-Zellen, Maus-Eierstockgewebe, Maushodengewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

17296-1-AP bindet in WB, IHC, IF/ICC, ELISA RPS4Y1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS4Y1 fusion protein Ag11176
• Vollständiger Name: ribosomal protein S4, Y-linked 1
• Berechnetes Molekulargewicht: 263 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC010286
• Gene symbol: RPS4Y1
• Gene ID (NCBI): 6192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS4Y1, a Y-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). RPS4Y maps to a 90-kb segment that has been implicated in Turner syndrome. As a homolog with RPS4X, the antibody can recognize endogenous level of RPS4Y and RPS4X, at 29-32kDa.

Publikationen

Species	Application	Title
human	IF	iScience; Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration; Authors - Fangbo Liu
human	WB	Discov Oncol; Construction of lung adenocarcinoma subtype and prognosis model based on fatty acid metabolism-related genes; Authors - Jing Chen