S6 Ribosomal protein Monoklonaler Antikörper

S6 Ribosomal protein Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(19)

CloneNo.

1C3E10

Rezensionen (1)

Kat-Nr. : 66886-1-Ig

Synonyme

RPS6, S6, 1C3E10, 40S ribosomal protein S6, OK/SW-cl.2

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• 

  WB analysis using 66886-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66886-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66886-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66886-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 66886-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66886-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66886-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 66886-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66886-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S6 Ribosomal protein antibody (66886-1-Ig, Clone: 1C3E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 66886-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human S6 Ribosomal protein (66886-1-Ig, Clone:1C3E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Rattenlebergewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Maus-Pankreasgewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IF: See 1 publications below

Produktinformation

66886-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA S6 Ribosomal protein und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S6 Ribosomal protein fusion protein Ag6599
• Vollständiger Name: ribosomal protein S6
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29-32 kDa
• GenBank-Zugangsnummer: BC000524
• Gene symbol: RPS6
• Gene ID (NCBI): 6194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publikationen

Species	Application	Title
human	WB	Genome Biol; A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.; Authors - Sooncheol Lee
human	WB	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	WB	Cell Mol Life Sci; Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling; Authors - Kunxiang Gong
human	IF	Microbiol Spectr; Structural Basis and Function of the N Terminus of SARS-CoV-2 Nonstructural Protein 1.; Authors - Kaitao Zhao
mouse	WB	Ecotoxicol Environ Saf; Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.; Authors - Yifan Hong
human	WB	Sci Rep; YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma.; Authors - Yuting Zhan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgane (Verified Customer) (09-15-2025)

Quite hard to detect on total protein extract, needs at least 50µg protein and longer exposure

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

S6 Ribosomal protein Antibody Western Blot validation (1/1000 dilution) in HCT116 (Cat no:66886-1-Ig)