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RSK2 Polyklonaler Antikörper
RSK2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 23762-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | NIH/3T3-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen |
Erfolgreiche IP | K-562-Zellen |
Erfolgreiche Detektion in IHC | humanes Nierengewebe, Maus-Kolongewebe, Mausnierengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Maushirngewebe |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, HUVEC-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:2000-1:5000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 8 publications below |
IHC | See 1 publications below |
CoIP | See 1 publications below |
Produktinformation
23762-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA RSK2 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | RSK2 fusion protein Ag20620 |
Vollständiger Name | ribosomal protein S6 kinase, 90kDa, polypeptide 3 |
Berechnetes Molekulargewicht | 740 aa, 84 kDa |
Beobachtetes Molekulargewicht | 84 kDa |
GenBank-Zugangsnummer | BC096301 |
Gene symbol | RSK2 |
Gene ID (NCBI) | 6197 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for RSK2 antibody 23762-1-AP | Protokoll herunterladen |
IHC protocol for RSK2 antibody 23762-1-AP | Protokoll herunterladenl |
IF protocol for RSK2 antibody 23762-1-AP | Protokoll herunterladen |
IP protocol for RSK2 antibody 23762-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Cancer Lett RSK2-mediated phosphorylation and degradation of UBE2O inhibits hepatocellular carcinoma growth and resistance to radiotherapy
| ||
Theranostics S6K1 phosphorylation-dependent degradation of Mxi1 by β-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer. | ||
Cell Death Differ β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer. | ||
Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma. | ||
Int J Biochem Cell Biol DHPAC, a novel synthetic microtubule destabilizing agent, possess high anti-tumor activity in vincristine-resistant oral epidermoid carcinoma in vitro and in vivo. | ||
Prostate MicroRNA181c inhibits prostate cancer cell growth and invasion by targeting multiple ERK signaling pathway components. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Iram (Verified Customer) (09-18-2025) | Very good antibody
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FH Morgane (Verified Customer) (09-15-2025) | Very clean band at expected size and a couple aspecific higher ones
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FH Iram (Verified Customer) (09-04-2020) | Very clean bands of RSK2 in immunoblotting
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