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  • KD/KO Validated

RSK2 Polyklonaler Antikörper

RSK2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 23762-1-AP

Synonyme

RPS6KA3, ISPK1, ISPK 1, Insulin-stimulated protein kinase 1, HU 3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBNIH/3T3-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen
Erfolgreiche IPK-562-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe, Maus-Kolongewebe, Mausnierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaushirngewebe
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:5000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

23762-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA RSK2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RSK2 fusion protein Ag20620
Vollständiger Name ribosomal protein S6 kinase, 90kDa, polypeptide 3
Berechnetes Molekulargewicht 740 aa, 84 kDa
Beobachtetes Molekulargewicht 84 kDa
GenBank-ZugangsnummerBC096301
Gene symbol RSK2
Gene ID (NCBI) 6197
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for RSK2 antibody 23762-1-APProtokoll herunterladen
IHC protocol for RSK2 antibody 23762-1-APProtokoll herunterladenl
IF protocol for RSK2 antibody 23762-1-APProtokoll herunterladen
IP protocol for RSK2 antibody 23762-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IHC,CoIP

Cancer Lett

RSK2-mediated phosphorylation and degradation of UBE2O inhibits hepatocellular carcinoma growth and resistance to radiotherapy

Authors - Yumei Huang
  • KD Validated

Theranostics

S6K1 phosphorylation-dependent degradation of Mxi1 by β-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer.

Authors - Yumei Huang
humanWB

Cell Death Differ

β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.

Authors - Jia Ma
humanWB

Oncogene

β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.

Authors - Yanwei Lu
humanWB

Int J Biochem Cell Biol

DHPAC, a novel synthetic microtubule destabilizing agent, possess high anti-tumor activity in vincristine-resistant oral epidermoid carcinoma in vitro and in vivo.

Authors - Ying Zhang
humanWB

Prostate

MicroRNA181c inhibits prostate cancer cell growth and invasion by targeting multiple ERK signaling pathway components.

Authors - Zhengzheng Su

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Iram (Verified Customer) (09-18-2025)

Very good antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: CRC cells
FH

Morgane (Verified Customer) (09-15-2025)

Very clean band at expected size and a couple aspecific higher ones

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HCT116
RSK2 Antibody Western Blot validation (1/1000 dilution) in HCT116 (Cat no:23762-1-AP)
FH

Iram (Verified Customer) (09-04-2020)

Very clean bands of RSK2 in immunoblotting

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 for western
  • Cell Tissue Type: Human colon cancer cells