RSK2 Polyklonaler Antikörper

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• KD/KO Validated

RSK2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 23762-1-AP

Synonyme

CLS, HU 3, ISPK 1, ISPK1, MAPKAP kinase 1b, MAPKAPK 1b, MAPKAPK1B, MRX19, p90 RSK 3, p90 RSK2, p90RSK3, pp90RSK2, Ribosomal S6 kinase 2, RPS6KA3, RSK, RSK 2, RSK2, S6K alpha 3, S6K alpha3

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 23762-1-AP

  WB result of RSK2 antibody (23762-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSK2 transfected K-562 cells.

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  WB analysis using 23762-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23762-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23762-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23762-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23762-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 23762-1-AP

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:4000) with K-562 cells lysate 3680 ug.

• 

  IP experiment of K-562 using 23762-1-AP

  IP Result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000ug.

• 

  IP experiment of K-562 using 23762-1-AP

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug.

• 

  IP experiment of K-562 using 23762-1-AP

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug.

• 

  IHC staining of human kidney using 23762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human kidney using 23762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody at dilution of 1:50(under 40x lens).

• 

  IF Staining of mouse brain using 23762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 23762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 23762-1-AP

  Immunofluorescent analysis of HUVEC cells using 23762-1-AP (RSK2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

23762-1-AP bindet in WB, IP, IHC, ELISA RSK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RSK2 fusion protein Ag20620
• Vollständiger Name: ribosomal protein S6 kinase, 90kDa, polypeptide 3
• Berechnetes Molekulargewicht: 740 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC096301
• Gene symbol: RPS6KA3
• Gene ID (NCBI): 6197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.

Publikationen

Species	Application	Title
		Theranostics; S6K1 phosphorylation-dependent degradation of Mxi1 by β-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer.; Authors - Yumei Huang
human	WB	Cell Death Differ; β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.; Authors - Jia Ma
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	Int J Biochem Cell Biol; DHPAC, a novel synthetic microtubule destabilizing agent, possess high anti-tumor activity in vincristine-resistant oral epidermoid carcinoma in vitro and in vivo.; Authors - Ying Zhang
human	WB	Prostate; MicroRNA181c inhibits prostate cancer cell growth and invasion by targeting multiple ERK signaling pathway components.; Authors - Zhengzheng Su
human	WB	PLoS One; A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction; Authors - Junwei Pan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (09-04-2020)

Very clean bands of RSK2 in immunoblotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Human colon cancer cells