RRBP1 Rekombinanter Antikörper

RRBP1 Rekombinant Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230159G12

Kat-Nr. : CL488-82890-2

Synonyme

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G12, 180 kDa ribosome receptor homolog

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  IF Staining of HepG2 using CL488-82890-2

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RRBP1 antibody (CL488-82890-2, Clone: 230159G12 ) at dilution of 1:200.

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  FC experiment of BxPC-3 using CL488-82890-2

  1x10^6 BxPC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated RRBP1 Recombinant antibody (CL488-82890-2, Clone:230159G12)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): BxPC-3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-82890-2 bindet in IF/ICC, FC (Intra) RRBP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RRBP1 fusion protein Ag16648
• Vollständiger Name: ribosome binding protein 1 homolog 180kDa (dog)
• Berechnetes Molekulargewicht: 1410 aa, 152 kDa
• Beobachtetes Molekulargewicht: 108 kDa, 152 kDa
• GenBank-Zugangsnummer: BC128577
• Gene symbol: RRBP1
• Gene ID (NCBI): 6238
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)