RRM1 Monoklonaler Antikörper

RRM1 Monoklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

4C12E10

Kat-Nr. : 60073-1-Ig

Synonyme

R1, ribonucleotide reductase M1, RIR1, RR1, RRM1

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 60073-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60073-1-Ig (RRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 60073-1-Ig

  IP Result of anti-RRM1 (IP:60073-1-Ig, 5ug; Detection:60073-1-Ig 1:1000) with K-562 cells lysate 3200ug.

• 

  IHC staining of human cervical cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 60073-1-Ig(RRM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 60073-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60073-1-Ig(RRM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 60073-1-Ig

  Immunofluorescent analysis of fixed HepG2 cells using 60073-1-Ig (RRM1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  FC experiment of HepG2 using 60073-1-Ig

  1X10^6 HepG2 cells were stained with .2ug RRM1 antibody (60073-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Urothelkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:3000-1:8000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

60073-1-Ig bindet in WB, IP, IHC, IF, FC,ELISA RRM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RRM1 fusion protein Ag0789
• Vollständiger Name: ribonucleotide reductase M1
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC006498
• Gene symbol: RRM1
• Gene ID (NCBI): 6240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B. RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).

Publikationen

Species	Application	Title
human	WB,IF	Cell Chem Biol; Clofarabine Commandeers the RNR-α-ZRANB3 Nuclear Signaling Axis.; Authors - Marcus J C Long
human	WB	Oncotarget; The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.; Authors - Lifei Fan
	IHC	World J Surg Oncol; A pilot study: sequential gemcitabine/cisplatin and icotinib as induction therapy for stage IIB to IIIA non-small-cell lung adenocarcinoma.; Authors - Lv Chao C