RSL24D1 Polyklonaler Antikörper

RSL24D1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 25190-1-AP

Synonyme

C15orf15, HRP L30 iso, L30, My024, Ribosomal L24 domain-containing protein 1

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  WB analysis of HepG2 using 25190-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 25190-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 25190-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25190-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 25190-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RSL24D1 antibody (25190-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HL-60-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 3 publications below

Produktinformation

25190-1-AP bindet in WB, IF/ICC, ELISA RSL24D1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RSL24D1 fusion protein Ag18195
• Vollständiger Name: ribosomal L24 domain containing 1
• Berechnetes Molekulargewicht: 163 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC008409
• Gene symbol: RSL24D1
• Gene ID (NCBI): 51187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RSL24D1 (Ribosomal L24 Domain Containing 1) is a ribosomal protein that is primarily localized in the nucleolus and is involved in the biogenesis of the 60S ribosomal subunit. In mouse embryonic stem cells (ESCs), the expression level of RSL24D1 is high and significantly decreases during cell differentiation. The role of RSL24D1 in ribosome biogenesis includes facilitating proper protein docking in nucleolar and cytoplasmic precursor particles. After the completion of biogenesis, RSL24D1 dissociates from the particles and is thought to be replaced by ribosomal protein L24.

Publikationen

Species	Application	Title
human	WB,IF	Cell Rep; Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly.; Authors - Chunyang Ni
human	WB	RNA; Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing; Authors - Mason A McCool
human	IF	mBio; Genome-Scale CRISPR Screening Reveals Host Factors Required for Ribosome Formation and Viral Replication; Authors - Maikke B Ohlson
human	WB	Cell; The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity; Authors - Vidhya Krishnamoorthy
human	WB,IF	Nat Cell Biol; A programmed decline in ribosome levels governs human early neurodevelopment; Authors - Chunyang Ni