RSPH3 Polyklonaler Antikörper

RSPH3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 17603-1-AP

Synonyme

RSP3, RSHL2, Radial spoke head-like protein 2, A-kinase anchor protein RSPH3

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  WB analysis of human brain using 17603-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17603-1-AP (RSPH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 17603-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17603-1-AP (RSPH3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 17603-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17603-1-AP (RSPH3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 17603-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17603-1-AP (RSPH3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of NIH/3T3 using 17603-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 17603-1-AP (RSPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Produktinformation

17603-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA RSPH3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RSPH3 fusion protein Ag11707
• Vollständiger Name: radial spoke 3 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 560 aa, 64 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC050604
• Gene symbol: RSPH3
• Gene ID (NCBI): 83861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
		J Mol Cell Biol; Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat.; Authors - Yirong Zhang
human	WB,IF	Andrology; Whole-exome sequencing identifies ADGB as a novel causative gene for male infertility in humans: from motility to fertilization; Authors - Yang Gao
mouse	IF	Sci Rep; Loss of Rsph9 causes neonatal hydrocephalus with abnormal development of motile cilia in mice.; Authors - Wenzheng Zou
human	IF	J Assist Reprod Genet; Patients with severe asthenoteratospermia carrying SPAG6 or RSPH3 mutations have a positive pregnancy outcome following intracytoplasmic sperm injection.; Authors - Huan Wu
mouse	CoIP	Nat Commun; Multi-scale structures of the mammalian radial spoke and divergence of axonemal complexes in ependymal cilia; Authors - Xueming Meng