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  • KD/KO Validated

RSPO3 Polyklonaler Antikörper

RSPO3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 17193-1-AP

Synonyme

CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, Protein with TSP type-1 repeat



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaus-Dünndarmgewebe, Transfizierte HEK-293-Zellen
Erfolgreiche IPhumanes Plazenta-Gewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17193-1-AP bindet in WB, IHC, IF, IP, ELISA RSPO3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RSPO3 fusion protein Ag9899
Vollständiger Name R-spondin 3 homolog (Xenopus laevis)
Berechnetes Molekulargewicht 272 aa, 31 kDa
Beobachtetes Molekulargewicht 30-40 kDa
GenBank-ZugangsnummerBC022367
Gene symbol RSPO3
Gene ID (NCBI) 84870
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for RSPO3 antibody 17193-1-APProtokoll herunterladen
IP protocol for RSPO3 antibody 17193-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nature

Hepatic stellate cells control liver zonation, size and functions via R-spondin 3

Authors - Atsushi Sugimoto
mouseWB

Nat Commun

R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine.

Authors - Teng Han
humanWB

Proc Natl Acad Sci U S A

RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.

Authors - Kendra S Carmon
mouseWB,IF

Cell Death Dis

Endothelial cell-derived RSPO3 activates Gαi1/3-Erk signaling and protects neurons from ischemia/reperfusion injury

Authors - Ting-Tao Liu
  • KD Validated
humanWB

Cardiovasc Res

Role of R-spondin 2 in arterial lymphangiogenesis and atherosclerosis.

Authors - Bhupesh Singla
ratWB

Heliyon

Astragalus polysaccharide improves diabetic ulcers by promoting M2-polarization of macrophages to reduce excessive inflammation via the β-catenin/ NF-κB axis at the late phase of wound-healing

Authors - Zhang Zhen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Gerald (Verified Customer) (07-23-2025)

Worked very well for Western Blot, showing a clear band at the expected molecular weight size, thereby confirming a successful transfection.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/250
RSPO3 Antibody Western Blot validation (1/250 dilution) in (Cat no:17193-1-AP)