RTEL1 Polyklonaler Antikörper

RTEL1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 25337-1-AP

Synonyme

Novel helicase like, NHL, KIAA1088, EC:3.6.4.12, C20orf41

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  WB analysis of BGC-823 using 25337-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 25337-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25337-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25337-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 25337-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25337-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 25337-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25337-1-AP

  IP result of anti-RTEL1 (IP:25337-1-AP, 4ug; Detection:25337-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IF Staining of HepG2 using 25337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTEL1 antibody (25337-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BGC-823-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Maushodengewebe, PC-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

25337-1-AP bindet in WB, IF/ICC, IP, ELISA RTEL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: regulator of telomere elongation helicase 1
• Berechnetes Molekulargewicht: 142 kDa
• Beobachtetes Molekulargewicht: 133 kDa
• GenBank-Zugangsnummer: NM_016434
• Gene symbol: RTEL1
• Gene ID (NCBI): 51750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
zebrafish	WB	Nucleic Acids Res; Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.; Authors - Jingzhen Li
human	WB	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang ; KD Validated
human	WB	J Clin Invest; CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes; Authors - Nunziata Maio
human	WB	J Cancer Res Clin Oncol; RTEL1 is upregulated in gastric cancer and promotes tumor growth; Authors - Chunyu Yang
human	WB	Cell Rep; A multimodal screening platform for endogenous dipeptide repeat proteins in C9orf72 patient iPSC neurons; Authors - Benedikt V Hölbling