RUFY2 Polyklonaler Antikörper

RUFY2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 19180-1-AP

Synonyme

ZFYVE13, RUN and FYVE domain-containing protein 2, RABIP4R, Rab4-interacting protein related, KIAA1537

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  WB analysis of mouse lung using 19180-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19180-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19180-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19180-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 19180-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19180-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Mauslungengewebe, Maus-Eierstockgewebe, Rattenlungengewebe, SH-SY5Y-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19180-1-AP bindet in WB, IHC, ELISA RUFY2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RUFY2 fusion protein Ag5938
• Vollständiger Name: RUN and FYVE domain containing 2
• Berechnetes Molekulargewicht: 403 aa, 46 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC041092
• Gene symbol: RUFY2
• Gene ID (NCBI): 55680
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

sarah (Verified Customer) (01-02-2025)

works well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000