RUVBL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RUVBL2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Rezensionen (1)

Kat-Nr. : 10195-1-AP

Synonyme

RUVB2, 48 kDa TATA box-binding protein-interacting protein, 48 kDa TBP-interacting protein, CGI-46, EC:3.6.4.12

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  WB analysis using 10195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10195-1-AP (RUVBL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 10195-1-AP

  IP result of anti-RUVBL2 (10195-1-AP for IP and Detection).

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  IP experiment of mouse brain using 10195-1-AP

  IP result of anti-RUVBL2 (IP:10195-1-AP, 4ug; Detection:10195-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10195-1-AP (RUVBL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RUVBL2 antibody (10195-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, Daudi-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Ratten-Blasengewebe, humanes Gliomgewebe, humanes Plazenta-Gewebe, Maus-Blasengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

10195-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RUVBL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RUVBL2 fusion protein Ag0253
• Vollständiger Name: RuvB-like 2 (E. coli)
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC000428
• Gene symbol: RUVBL2
• Gene ID (NCBI): 10856
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.; Authors - Sang Gyun Kim
human	WB,IF	Sci Adv; Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.; Authors - Seung Ho Shin ; KD Validated
human	WB,IP	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
mouse	IP	J Clin Invest; circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.; Authors - Zhenzhen Chen
human	WB	Sci Adv; ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer.; Authors - Franklin C Harwood
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

It worked well in H1 human embryonic stem cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human embryonic stem cell

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